Caracterização físico-química de lacase do camarão litopenaeus vannamei e o uso dessa enzima para a degradação de corantes

Abstract

Aiming at bioremediation to combat impacts caused by textile effluents, the present study aimed to determine the physicochemical parameters of the enzyme and to evaluate the effect of laccases from the main shrimp species cultivated in Brazil (Litopenaeus vannamei). Thus, with the enzyme already partially purified, the determination of the activity of laccases was carried out, using the substrate ABTS and Choline, later, the physicochemical parameters were determined, and finally, the effect of laccase enzymes on the degradation of dyes was analyzed. phenol red, coomassie brilliant blue r, bromophenol blue and methylene blue. With this, the enzymes already partially purified, together with ABTS, it can be observed that it did not obtain specificity with the substrate, thus, there was no activity. With this, the substrate Colina was used to determine the physicochemical parameters, where it was able to react with the enzyme and presented an optimal alkaline pH (pH 9.5) and obtained its optimal temperature at 100°C. After that, the percentage of degradation was calculated through the absorbance per minute, in the absence and in the presence of laccase, obtaining a satisfactory result for the degradation of the bromophenol blue dye. Thus, laccase was able to degrade one of the dyes, for future studies which will allow use laccases as an important enzyme against dyes.