Quantificação, identificaçãoe bioprospecção de fungos cultiváveis, de solo em recuperação, no semiárido Pernambucano

Abstract

Oil microbial communities suffer interference from biotic and abiotic factors, and in the case of semi-arid, these communities are subject to low water availability, high temperatures andhigh incidence of solar radiation. These conditions require high adaptation of these organisms, which may represent an important source of metabolites of interest to biotechnology. In this way, this work aimed at the study of the dynamics of the fungal community of soil in recovery of the semi -arid Pernambucano collected in the Conservation Unit State Park Mata da Pimenteira, in the municipality of Serra Talhada -PE. In order to do so, the soils were collected in two seasonal periods (one in the rainy season and two in the dry season) and the isolation and quantification of total fungi and thermophilic, osmotic and halophilic fungi were carried out.Antimicrobial and enzymatic activity tests of these fungi were also carried out. In collection 1 (rainy season) the highest quantification was obtained with 2.3 x 10⁷ CFU / mL of mesophilic fungi; from the same collection it was possible to isolate all groups. In collections 2 and 3 (drought period), the highest quantification was 7.33 x 10⁶ and 5.0 x 10⁶ CFU / mL of osmotic and mesophilic fungi, respectively. There was no isolation of halophilic fungi in samples 2 and 3. Of the 22 isolates selected and submitted to the test of antimicrobial activity, the isolate 11 (Fusarium sp.) With a halo of 8.33 mm in diameter was distinguished from Bacillus subtilis. Eighteen of the twenty fungi tested showed enzymatic activity with degradation of at least one tested substrate. These results allow establishinga correlation between the characteristics of the soils and the areas where they were collected, with the microbial populations found, besides valuing the biotechnological diversity and potential of the isolated microorganisms.