Relatório de Estágio Supervisionado Obrigatório (ESO) realizado no Laboratório de Ecofisiologia e Comportamento Animal (LECA-UFRPE), Recife-PE

Abstract

Due to the importance of the veterinarian in zebrafish (Danio rerio) vivarium, the Estágio Supervisionado Obrigatório (ESO) was carried out during the period from September 20 to December 07, 2018, in the Laboratório de Ecofisiologia e Comportamento Animal (LECA) of the Departamento de Morfologia e Fisiologia Animal (DMFA) of the Universidade Federal Rural de Pernambuco (UFRPE) in Dois Irmãos, Recife, Pernambuco, Brazil. The report was divided into Chapter I, describing the stage entity and the activities developed, depicting the training developed at the stage, and Chapter II, which represents the experiment performed. Zebrafish has become internationally recognized as an experimental model for evaluating acute and chronic toxicity tests caused by chemical products in scientific research in recent decades. Serjania glabrata Kunth is a plant present in the Caatinga biome widely distributed in the Semiarid region of Northeastern Brazil and has been reported as toxic to goats by producers. Thus, this study aimed to evaluate the toxic effects of the ethanolic extract of the leaves of Serjania glabrata Kunth on zebrafish embryos (Danio rerio). The leaves of S. glabrata Kunth were collected in the county of Salgado de São Félix-PB and, from these, the ethanolic extract diluted in 1% dimethylsulfoxide with a concentration of 5 mg/mL was obtained. The parameters evaluated were heart rate, mortality and teratogenic effects, such as column and tail deformation, pericardial and vitelline sac edema, and coagulation, at 24, 48, 72 and 96 hours post fertilization intervals under an optical microscope. The concentrations of 10, 100 and 1000 μg/L did not cause teratogenic effects, as well as mortality, but caused alterations in heart rate.