Estudo do polimorfismo +874 T/A do gene interferon gamma em pacientes com artrite reumatoide do estado de Pernambuco

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease with prevalence between 0.5% and 1% of the adult population worldwide. The etiology is still not fully understood, but it is known that hormonal, environmental and immunological factors act together in individuals with genetic predisposition. Among the genetic factors, Interferon Gamma (IFNG) +874 T/A gene polymorphism is cited as acting in several autoimmune diseases. In this sense, the objective of this work is to analyze the relation of the +874 T / A polymorphism of the IFNG gene in RA, in a population of the State of Pernambuco. The present study was composed of 127 patients and 127 healthy controls, from which the blood samples collected were used for DNA extraction. Subsequently, amplification of the extracted DNA was performed, using the allelespecific polymerase chain reaction (AS-PCR). The amplified material was subjected to 2% agarose gel electrophoresis. Statistical calculations were applied using Chi-square and Analysis of Variance. The results indicated that the + 874 T / A polymorphism of the IFNG gene is not associated to the development of RA, with genotypic distribution (TA x AA: p = 0.530 and TT x AA: p = 0.416) and allelic frequency (p = 0.851). However, individuals with TT and TA genotypes had significantly higher rates of disease activity compared to the AA genotype (DAS28: p = 0.017; CDAI: p = 0.021). Suggesting that the +874 T/A polymorphism of the IFNG gene can be considered as a marker for RA disease activity.