Infecção do trato urinário por Proteus mirabilis em cão paraplégico atendido em um hospital veterinário escola de Recife - PE

Abstract

The Mandatory Supervised Internship (MSI) of the Veterinary Medicine course at UFRPE is essential for students to explore the professional environment before graduating. Required to obtain a bachelor's degree, the internship provides the development of theoretical and practical skills acquired throughout academic training. The MSI is important to train undergraduates in technical and social skills, facilitating their integration into the job market. At UFRPE, the internship was carried out at the Laboratory of Infectious Diseases of the Veterinary School Hospital, totaling 420 hours, taking place from April 1 to June 14, 2024. The laboratory is equipped with adequate infrastructure for microbiological analysis, including benches, microscopes, incubators, refrigerators and laminar flow hoods. During the internship, the focus was on the diagnosis of infections, such as bacteriuria by Proteus mirabilis in a dog, establishing a relationship among clinical, biochemical findings and antimicrobial sensitivity tests. The report therefore covers the description of the activities carried out in the laboratory, including the work routine, exam casuistry and that specific case study. This experience allows Veterinary Medicine students to envision their future professional careers in various specialized areas, contributing to a more complete education and preparation for the challenges of the profession. The Lab’s mission is to isolate and identify pathogenic microorganisms in biological samples from animals, providing support to the veterinary medical clinic. The main activities include the identification and isolation of bacteria and fungi. In addition, the laboratory performs support tasks, such as reprocessing instruments, sterilizing materials, preparing culture media and performing biochemical tests. The biological samples received include blood, urine, tissues, biological fluids and swabs from lesions, otological or ophthalmological, from the hospital or from external sources. Upon reception, each sample follows a specific flowchart, differentiating between bacteriological and mycological tests to avoid cross-contamination. For bacteriological examinations, samples are processed on specific media dishes, streaked and incubated at 37ºC. After growth, colonies are analyzed by Gram staining and biochemical tests for identification. Mycological examinations include direct examination and fungal culture on Mycosel agar. The laboratory also performs antibiograms to determine antimicrobial sensitivity, following CLSI and BrCAST guidelines. During the internship, 141 procedures were performed, with a predominance of canine and feline samples, as well as birds and wild animals. These activities are essential to provide accurate diagnoses and effective therapy, contributing to animal health and veterinary practice.