Detecção de anticorpos anti-Leptospira spp. em asininos (Equus asinus) do estado de Pernambuco - Brasil

Abstract

The objective of this work was to describe the activities carried out during the mandatory supervised internship,conducted at the Veterinary Bacteriology Laboratory (LaBV) of the Federal Fluminense University (UFF). The internship took place at the Biomedical Institute of UFF, located on Alameda Barros Terra Street, in São Domingos, Niterói-RJ, under the supervision of Prof. Dr. Walter Lilenbaum and guidance from Prof. Dr. Erika Fernanda Torres Samico-Fernandes, during the period from 10/2/2023, to 12/22/2023. It was possible to observe the routine activities of LaBV, conducting research on Leptospirosis, performing routine serological tests using the Microscopic Agglutination Test (MAT) technique, and molecular biology research using the Polymerase Chain Reaction (PCR) technique. Additionally, I had the opportunity to observe the culture and maintenance of samples containing live Leptospira spp. Additionally, it was possible to participate in the research activities conducted in the laboratory, leading to the preparation of a scientific article aimed at detecting the presence of anti-Leptospira spp. antibodies in samples from donkeys originating from two properties in the state of Pernambuco, Brazil, where the Australis serogroup was the most commonly detected.