Efeito da adição de zeólita natural (Clinoptilolita) em dietas sobre os parâmetros sanguíneos de poedeiras

Abstract

The objective of this work is to evaluate the effect of the addition of natural zeolite (Clinoptilolite) in diets on the serum and hematological biochemical profile of laying hens. The treatments consisted of six levels of zeolite inclusion, 0, 0.7; 1.4; 2.1; 2.8; 3.5%, where all diets were isoenergetic and isoproteic, differing only the replacement of an inert material (washed sand) by the zeolite additive. The design was in randomized blocks, with 8 replications and 5 birds per plot, totaling 240 ISA-Brown laying hens from the 54th week with approximate weight of 1.75 kg. To perform the hematological analysis, 1 bird per plot was used and 4ml blood samples were collected from the ulnar vein of one of the wings. The hematological variables were: red blood cells, hemoglobin, platelets, leukocytes, total plasma proteins, heterophils, lymphocytes, eosinophils and monocytes. To evaluate the biochemical parameters, samples of 4ml of blood each were collected from 2 birds per plot and submitted to analysis of total proteins, albumin, calcium, creatinine, urate/uric acid, urea, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT). Data were submitted to analysis of variance, and if significant, regression analysis and Dunnet's test at 5% probability were performed. No significant effect was observed for the biochemical parameters. As for the hematological variables, an effect of zeolite on total lymphocytes was observed (p=0.0006). When the regression analysis was performed, a quadratic effect was found (p=0.0035). When applying Dunnet's test, it was found that the absolute value of lymphocytes differed as a function of natural zeolite (Clinoptilolite) in relation to the control diet at the level of 3.5% inclusion. With this, it is concluded that clinoptilolite can be used in the feeding of laying birds at the level of 3.5% of inclusion, providing a greater amount of lymphocytes, stimulating the production of antibodies and improving immunity.