Criopreservação de sêmen de peixe de águas doces tropicais: uma revisão tecnológica

Abstract

The aim of this article is to identify the techniques used in cryopreservation of fish semen. The methodology used was the integrative review of articles on the topic researched on Capes' Journal Portal and nine articles were chosen to be analyzed, as they contained all the information analyzed by the researcher. A table was also developed which contains a summary of the technique used in cryopreservation, in particular the biotechnological aspects, such as: diluents, cryoprotectants, packaging, freezing, storage, thawing and the activating solution. A mental map of the cryopreservation technique was developed, from fresh semen preparation to thawing and eventual use. The bibliography analysis shows that glucose is the most used diluent, together with Dimethylsufoxide - DMSO, as an internal cryoprotectant, the product is packaged in straws or microtubes, freezing is mainly done using a Dry Shipper and subsequent storage in liquid nitrogen (-196ºC) and the thawing is done quickly and for a short time and the subsequent activation is done with sodium chloride or sodium bicarbonate. It is concluded that the technique is well described and there is already a protocol for the development of cryopreservation.