Fungos endofíticos em Bauhinia cheilantha (Bong.) Steud. Leguminosae, no Semiárido pernambucano

Abstract

Endophytic fungi are microorganisms that are present in plants and can colonize inter or intracellular various organs, establishing a mutualist relationship with the host. They can be associated with different plant species, including medicinal ones, producing secondary metabolites of biotechnological interest such as antibiotics. Therefore, the objective of this work was to isolate and taxonomically identify the endophytic fungi present in leaves of Bauhinia cheilantha (cowpaw, mororó) collected in Pernambuco semiarid, as well as to evaluate compound with antimicrobial potential. After random leaf collection and disinfection, 140 fragments were removed to isolate the endophytes in 20 Petri dishes containing Sabouraud Agar medium. Of these, 10 plates were kept at room temperature and 10 were stored in a greenhouse at 30 ° C for five days. Subsequently, isolation peaks were performed in pure culture for seven days. Thirty isolates of endophytic fungi were obtained, of which 17 were submitted to the antimicrobial activity test in Müeller Hinton agar solid medium against potentially pathogenic bacteria. No antimicrobial activity was observed under the tested conditions, however, five genera were identified: Alternaria sp., Bipolaris sp., Colletotrichum sp., Nigrospora sp. and Drecheslera sp., which constitute the first report, so far, endophytic fungi present in B. cheilantha for Pernambuco semiarid.