Relatório De Estágio Supervisionado Obrigatório (ESO), realizado no Center For Conservation And Research Of Endangered Wildlife - CREW no Zoológico De Cincinnati (OHIO, US) e na Associação Mata Ciliar - AMC Em Jundiaí (SP)

Abstract

Due to environmental impacts, a lot of species are suffering from loss habitat, loss of populations and genetic diversity. One alternative to minimize loss of population and genetic diversity it is by using Reproductive Biotechniques. These techniques allow new individuals to be generated and also several samples of different genetic sources be stored for future use. The internship accomplished at The Center for Conservation and Research of Endangered Wildlife and at Mata Ciliar Association looked for improve skills in the area of assisted reproduction in felines, with focus on neotropical felines. Using the domestic cat (Felis catus) as a model, it was possible to perform several evaluations and methodologies linked to Reproductive Biotechnics such as morphological evaluation, conventional slow freeze, ultra rapid freeze, oocyte maturation, in vitro fertilization and embryo production. After learning those methodologies, it was possible to develop a seminary related, being possible to collect two individuals of cougar using the techniques of urethral catheterization and Electroejaculation and freeze the ejaculate of one specimen. Other activities could also be done, such as surgery and laparoscopy, clinical attendance of rescued wildlife, conditioning, maintenance of enclosures and educational activities. The experiences during the internship period allowed the acquisition of new skills and also the improvement of others, being really important for the professional career of the intern.